Insulin Increases Sestrin 2 Content by Reducing Its Degradation through the PI₃K/mTOR Signaling Pathway

Figure 4

SESN2 siRNA knockdown enhances insulin signaling by repressing the PTEN level. HepG2 cells were transfected with either the control or the SESN2 siRNA for 48–72 h as indicated in Methods. (a) The cells were harvested for western blotting with indicated SESN antibodies. (b) and (c), HepG2 cells were transfected with either the control or the SESN2 siRNA for 48–72 h. The cells were then serum-starved for 4 h, followed by 100 nM insulin treatment for 15 min. Western blotting was performed with indicated antibodies. Right panels show the results of densitometric analysis. compared with noninsulin treatment.

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