Effects of Ghrelin on Triglyceride Accumulation and Glucose Uptake in Primary Cultured Rat Myoblasts under Palmitic Acid-Induced High Fat Conditions

Figure 5

Effects of acylated ghrelin on the mRNA expression of genes involved in lipid and glucose metabolism. mRNA expression of the glucose transporter 4 (GLUT4), AMP-activated protein kinase (AMPK), and uncoupling protein 3 (UCP3) in primary cultured rat myoblasts under high fat conditions was determined by real-time polymerase chain reaction. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as internal reference. Data are shown as mean ± SD. , versus controls; versus the 0.3 mM PA group.