Functional Diversity of Fibroblast Growth Factors in Bone Formation

Figure 3

1,25(OH)₂D₃ increases Fgf9 and Fgf23 gene expression at late development stages in rat calvaria cell cultures. Rat calvaria cells were obtained as shown in Figure 2. At day 11, nodule-forming cells were stripped by collagenase and replated (subcultures). Four days later, osteoblast subcultures were pretreated with or without actinomycin D (ActD) or cycloheximide (CHX), followed by incubation with 1 nM 1,25(OH)₂D₃ for 6 h. See the above mentioned for qPCR. Data represent means ± S.D. . Statistical significance of differences was analyzed with one-way or two-way analysis of variance (ANOVA) with repeated measures, followed by Tukey’s multiple comparison test. versus vehicle alone; versus 1,25(OH)₂D₃ alone.