Modulation of Insulin Sensitivity of Hepatocytes by the Pharmacological Downregulation of Phospholipase D

Figure 4

Effects of 1-butanol on phospholipase D activity and glucose metabolism stimulation by insulin in the isolated hepatocytes. (a) Phospholipase D activity, (b) uptake of 2-D-[³H]glucose, (c) incorporation of D-[U ¹⁴C]glucose into glycogen. Effect of insulin on phospholipase D activity and glucose metabolism in the 1-butanol- or 2-butanol-pretreated hepatocytes was determined as described in the Methods. , insulin versus control; , insulin + 1-butanol versus insulin + 2-butanol; , 1-butanol versus 2-butanol.

(a)

(b)

(c)