Modulation of Insulin Sensitivity of Hepatocytes by the Pharmacological Downregulation of Phospholipase D

Figure 6

Effect of halopemide and ceramide on phospholipase D activity and glucose metabolism stimulation by insulin in the isolated hepatocytes. (a) Phospholipase D activity, (b) uptake of 2-D-[³H]glucose, (c) incorporation of D-[U ¹⁴C]glucose into glycogen. Effect of insulin on phospholipase D activity and glucose metabolism in the halopemide or C6-ceramide-pretreated hepatocytes was determined as described in the Methods. , insulin versus control; , insulin + drug versus insulin; , C6-ceramide in basal condition versus basal condition, control cells.

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