Research Article

Effect of Advanced Glycation End Products on Human Thyroglobulin’s Antigenicity as Identified by the Use of Sera from Patients with Hashimoto’s Thyroiditis and Gestational Diabetes Mellitus

Figure 2

Analysis by SDS-PAGE of AGE-modified-Tg (in the presence of glucose, with pH adjustment at 7.4). SDS-PAGE analysis of native Tg and AGE-Tg produced after incubation of Tg with two different concentrations of D-glucose-6-phosphate (pH adjusted at 7.4). Samples (10 μg) were analyzed in a 7.5% separating gel under denaturing, nonreducing conditions and then stained with Coomassie blue. Lane 1: molecular weight markers (94, 67, 43, and 30 KDa), lane 2: native Tg (330 KDa), lane 3: AGE-Tg produced with 0.03 M D-glucose-6-phosphate, and lane 4: AGE-Tg produced with 0.004 M D-glucose-6-phosphate.