Effect of Advanced Glycation End Products on Human Thyroglobulin’s Antigenicity as Identified by the Use of Sera from Patients with Hashimoto’s Thyroiditis and Gestational Diabetes Mellitus

<div>Titration curves of pathological (GDM and HT) and normal control sera against native Tg and low or high AGE-Tg. Threefold serial dilutions (1/50, 1/150, and 1/450) of 20 sera from pregnant women with GDM (open squares), 20 sera from women with HT (black squares), and 20 sera from healthy control women (black circles) were tested for antibody reactivity against native Tg (0,78 AGE units/mg Tg) (a), low AGE-Tg (13 AGE units/mg Tg) (b), and high AGE-Tg (49 AGE units/mg Tg) (c) by an in-house ELISA. Antibody reactivities were detected using alkaline phosphatase-conjugated anti-human IgG antibody and the adequate chromogenic substrate; mean OD values and SD (error bars) of sera from each group were calculated.</div>

International Journal of Endocrinology

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Figure 3: Effect of Advanced Glycation End Products on Human Thyroglobulin’s Antigenicity as Identified by the Use of Sera from Patients with Hashimoto’s Thyroiditis and Gestational Diabetes Mellitus 

Figure 3 | Effect of Advanced Glycation End Products on Human Thyroglobulin’s Antigenicity as Identified by the Use of Sera from Patients with Hashimoto’s Thyroiditis and Gestational Diabetes Mellitus 