Methylation of the Corticotropin Releasing Hormone Gene Promoter in BeWo Cells: Relationship to Gene Activity

Figure 2

Methylation of the CRH promoter. Percent methylation of the 9 CpG sites together was calculated by combining three independent experiments and ten randomly selected clones (individual gene copies) sequenced per treatment in each experiment. Methylation frequencies were compared by Fisher’s exact test. Statistical comparisons (, significant and NS, not significant) are shown by the arrows. 0 h, no treatment; 72 h, 72 h of culture; cAMP 72 h, treatment with 2.5 × 10⁻⁴ mol L⁻¹ of 8-Br-cAMP for 72 h; AZA 72 h, treatment with 5 × 10⁻⁶mol L⁻¹ 5-AZA-dC for 72 h; cAMP + AZA 72 h, combined treatment with 8-Br-cAMP and 5-AZA-dC for 72 h.