Paraoxonase 2 Induces a Phenotypic Switch in Macrophage Polarization Favoring an M2 Anti-Inflammatory State

Figure 5

Direct effect of hPON2 on M1 functional phenotype in PON2KO MPM. MPM obtained from 3-month-old PON2KO male mice were transfected with pcDNA3.1+ empty plasmid (EP) or with pcDNA3.1+ plasmid containing hPON2 for 4 hours at 37°C. Cells were then washed and cultured with DMEM medium + 10% fetal calf serum for 48 hours. The cells were cultured for an additional 12 hours either unstimulated or stimulated with IFNγ/LPS or IL-4. (a) Phagocytosis was determined in cells that were incubated with FITC-conjugated latex beads for an additional 4 hours and analyzed using FACS. Results are given as mean fluorescence intensity (MFI). (b) ROS production was measured after 45 minutes of incubation with DCFH by FACS and is expressed as MFI. Results are expressed as mean ± SD (), , PON2KO MPM versus control MPM; , M1 stimulated versus unstimulated cells.

(a) Phagocytosis

(b) ROS production