Ghrelin Protects against Dexamethasone-Induced INS-1 Cell Apoptosis via ERK and p38MAPK Signaling

Figure 2

Effects of ghrelin and dexamethasone on DNA synthesis in INS-1 cells. (a) INS-1 cells were cultured in complete medium (control), 0.1 μM dexamethasone, 0.1 μM ghrelin, and 0.1 μM dexamethasone + 0.1 μM ghrelin for 48 h. EdU-labeled INS-1 cells were assessed using fluorescent microscopy. All cell nuclei were Hoechst 33342-positive (blue) and all replicating cells were EdU-positive (red). (b) The graph represents the percentage of EdU-labeled proliferative INS-1 cells. Data are presented as the mean ± SD. versus control group; versus dexamethasone group.

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