Research Article

Sex Hormone Binding Globulin Modifies Testosterone Action and Metabolism in Prostate Cancer Cells

Figure 4

Effects of testosterone (Te) and Sex Hormone Binding Globulin (SHBG) on prostate specific antigen (PSA), androgen receptor (AR), and SHBG mRNA expression in LNCaP cells by quantitative Real-Time PCR. (a) PSA mRNA expression in cells treated with 0, 1, 10, and 25 nM Te ± 25 nM SHBG for 24 hours. (b) PSA mRNA expression in cells treated with 25 nM SHBG plus 0, 1, 5, 10, or 25 nM Te. Expression of PSA (c), AR (d), and SHBG (e) mRNA in LNCaP cells treated with 10 nM Te plus 0, 10, 25, or 50 nM SHBG for 24 hours. All experiments were performed at least three times in triplicate. The mRNA expression was normalised to the housekeeping gene, β2-microglobulin (B2M). One-way ANOVA with Tukey’s test was used for statistical analysis. Tukey’s test indicated as “no significance” (ns) or asterisk (; ; and ). All results were represented as mean ± standard error (SEM).
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