Sex Hormone Binding Globulin Modifies Testosterone Action and Metabolism in Prostate Cancer Cells

Table 1

Primer sequences for quantitative RT-PCR.

Gene name	Primers	Location	GenBank accession number
PSA	(F) 5′-GTGTGTGGACCTCCATGTTATT-3′; (R) 5′-TGCCCCATGACGTGATACCT-3′	516-537; 691-672	NM_001648
AR	(F) 5′-GGCTGTCATTCAGTACTCCTG-3′; (R) 5′-GG AGC CAT CCA AAC TCT TGA-3′	3320-3340; 3491-3510	NM_000044
SHBG	(F) 5′-GCCCAGGACAAGAGCCTATC-3′; (R) 5′-CCTTAGGGTTGGTATCCCCATAA-3′	149-168; 277-255	NM_001146281
B2M	(F) 5′-GGCTATCCAGCGTACTCCAAA-3′; (R) 5′-CGGCAGGCATACTCATCTTTTT-3′	57-77; 302-281	NM_004048

Reference: http://pga.mgh.harvard.edu/primerbank/index.html.
(F): forward; (R): reverse; PSA: prostate specific antigen; AR: androgen receptor; SHBG: sex hormone binding globulin.