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Figure 2: Results from Dicer1 siRNA transfection of H295R adrenocortical cells. Cells were transfected with Dicer1 siRNA or with a scrambled negative-control siRNA (mRNA levels for StAR (a), CYP11A1 (b), 3βHSD11 (c), CYP21A (d), CYP17A1 (e), and HSD11B2 (f) were analysed 48 hours posttransfection by qRT-PCR). Cycle threshold values were normalised to β-actin mRNA and expressed relative to control cell levels. Results represent the mean of three independent biological experiments performed in triplicate ± SEM; , compared to scrambled control.