Regulation of Corticosteroidogenic Genes by MicroRNAs
(a–d) Results from HeLa cells transfected with pEZX-B2 3′UTR reporter construct plasmids and either a specific miRNA Pre-miR or Anti-miR, or with a scrambled negative control RNA. Firefly and renilla luciferase luminescence was measured 48 hours posttransfection, and results are presented as firefly reporter gene luminescence normalised for renilla luminescence. H295R cells transfected with either miR-125a-5p (e) or miR-125b-5p (f) Pre-miR, miR-24 Anti-miR, or with a scrambled negative control RNA. CYP11B2 mRNA was measured 48 hours posttransfection by qRT-PCR, with cycle threshold values normalised to GAPDH mRNA and expressed relative to negative control. Results represent the mean of three independent biological experiments performed in triplicate ± SEM; , compared to negative control.