Figure 5: H295R cells transfected with miR-320a-3p Pre-miR, miR-320a-3p Anti-miR, or with a scrambled negative control RNA. CYP11A1 (a) or CYP17A1 (b) mRNA was measured 48 hours posttransfection by qRT-PCR, with cycle threshold values normalised to β-actin mRNA and expressed relative to negative control. Results represent the mean of three independent biological experiments performed in triplicate ± SEM; and compared to negative control.