Research Article

Androgen Receptor Localizes to Plasma Membrane by Binding to Caveolin-1 in Mouse Sertoli Cells

Figure 1

Testosterone induced membrane association of AR. (a) Representative Western blot analysis of AR levels in cytoplasmic, membrane, and nuclear proteins from cells incubated for 30 min with vehicle (control (C)) or 10 nM testosterone (T). (b) Semiquantitative analysis of AR levels in the subcellular fractions after exposure of the cells to vehicle (basal) or testosterone for 30 min. Data were expressed as the mean and SEM of the ODs of the AR band, multiplied by the testosterone-to-basal ratio of the respective mark proteins, GAPDH, HDAC, and pan-cadherin for cytoplasmic, nuclear, and membrane fractions. compared with that of the respective basal (). Confocal microscopy imaging of the effect of testosterone on AR trafficking. Cells were incubated with 10 nM testosterone for 30 min, before fixation and confocal microscopy examination. Bar = 50 μm.
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