Muscarinic Receptors Types 1 and 2 in the Preoptic-Anterior Hypothalamic Areas Regulate Ovulation Unequally in the Rat Oestrous Cycle

Figure 3

Number of m₁AChR or m₂AChR immunostained neurons (m₁AChR-ir or m2AChR-ir) in the POA-AHA region throughout the oestrous cycle in the rat. A number of m₁AChR-ir (a) or m₂AChR-ir (b) were counted on both sides (left side plus right side) of the medial preoptic nucleus in the preoptic region at 9:00 (grey bar), 13:00 (blue bar), or 17:00 h (green bar) for each phase of the oestrous cycle. The results are expressed as the mean ± SEM. versus 9:00 h, for its respective phase of the oestrous cycle. D1, dioestrous-1; D2, dioestrous-2; P, proestrous; E, oestrous.

(a)

(b)