Activities of Amphioxus GH-Like Protein in Osmoregulation: Insight into Origin of Vertebrate GH Family

Figure 3

Induction of nka and nkcc expressions by rGHl. (a) Expression of nka in the gill cultures in response to rGHl or rzGH (concentrations ranging from 10 ng/ml to 1000 ng/ml). (b) Expression of nkcc in the gill cultures in response to rGHl or rzGH (concentrations ranging from 10 ng/ml to 1000 ng/ml). (c) Expression of nka in the gill of amphioxus injected with saline, rGHl, or rzGH. (d) Expression of nkcc in the gill of amphioxus injected with saline, rGHl, or rzGH. (e) NKA activity in the gill of amphioxus injected with saline, rGHl, or rzGH. One unit of enzymatic activity was defined as 1 μmoles ADP released per hour. Data were from 3 independent experiments and expressed as mean ± SEM. The symbol shows .

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