Simulated Cholinergic Reinnervation of β (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing β Cell and Cholinergic Cell

Figure 6

E-cadherin plays a key role in heterotypic pseudoislet formation. (a) E-cadherin was detected in cell climbing slices by indirect immunofluorescence (40x). (b) E-cadherin was detected in heterotypic pseudoislets by indirect immunofluorescence (20x). (a and b) Red: NG108-15 cells, green: INS-1 cells, and blue: E-cadherin. Scale bars: 50 μm. (c) E-cadherin was detected in INS-1 and NG108-15 cells by Western blot. Lane 1: 2D-cultured INS-1 cells, lane 2: 3D-cultured INS-1 cells, lane 3: 2D-cultured NG108-15 cells, and lane 4: 3D-cultured NG108-15 cells. versus 2D INS-1 group and versus 2D NG108-15 group, . (d) Number of heterotypic pseudoislets formed per well. INS-1 and NG108-15 cells were seeded into 96-well ultra-low attachment plates and maintained in coculture medium (control) with or without E-cadherin antibody or β actin antibody for 24 hours. ns versus control group. versus anti-β-actin group, .

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