GnRH Antagonist Improves Pubertal Cyclophosphamide-Induced Long-Term Testicular Injury in Adult Rats

Figure 2

Representative photomicrograph of AR immunochemistry and Western blot assays in the testis. (a) (A) The control group showed strong AR immunostaining in the Sertoli, Leydig, and peritubular myoid cells. (B) The CPA group showed markedly decreased AR expression. (C) The GnRHant + CPA group showed relatively restored AR expression. (D) The GnRHant group showed the same strong AR expression as the control group. (A, B, C, and D ×400). (b) Quantitative analysis of AR expression by immunohistochemistry in the testis. Significantly different from the other three groups (). Much higher than that in the CPA group (). (c) Representative photographs of Western blot for AR protein expression. (d) Quantitative analysis of AR expression by Western blot in the testis. compared with the other three groups (). , compared with the GnRHant + CPA group.

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