Beta-Arrestin 1 Mediates Liver Thyrotropin Regulation of Cholesterol Conversion Metabolism via the Akt-Dependent Pathway

Figure 1

TSH influences transcriptional and translational levels of β-arrestins. Liver tissues of SCH mice and wild-type counterparts, which have the same serum free T4 level but different TSH level, were extracted, and mRNA transcriptional analysis of β-arrestins was performed through qRT-PCR. Protein samples of HepG2 cells treated with 4 μM TSH were extracted in a time course and analyzed by Western blot. (a) TSH and free T4 level of SCH mice and control individuals. (b) Relative mRNA level of β-arrestins in the liver of SCH mice and control individuals. (c, d) Immunoblotting analysis of β-arrestin expression in HepG2 cells under 4 μM TSH stimulation in 2 and 24 hours. (e, f) Grayscale analysis of β-arrestin expression in HepG2 cells under 4 μM TSH stimulation for 2 and 24 hours. All experiments were repeated 3 times with similar results. The differences between groups were compared by using nonparametric tests. versus control group. versus control group. Error bars are calculated as a standard error (SEM).

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