Beta-Arrestin 1 Mediates Liver Thyrotropin Regulation of Cholesterol Conversion Metabolism via the Akt-Dependent Pathway

Figure 4

GloSensor analysis of cAMP level in ARRB1/2-knockdown HepG2 cells under TSH treatment. The cells were maintained in culture medium for 24 hours; then, the medium was changed with 100 μL of CO₂-independent medium with 2% v/v GloSensor cAMP Reagent (E1171, Promega) and 10% FBS for two hours. The cAMP signal was initiated by adding TSH, with forskolin (10 μM) and PBS as positive and negative controls, respectively. GloSensor luciferase activities were measured on a microplate luminometer (Berthold Centro XS³ LB960) for 2 hours. For each treatment, 8 wells of cells were used as duplicated samples. For statistical analysis, . Error bars are calculated as a standard error (SEM). . Error bars are calculated as a standard error (SEM).