Research Article

Beta-Arrestin 1 Mediates Liver Thyrotropin Regulation of Cholesterol Conversion Metabolism via the Akt-Dependent Pathway

Figure 7

Immunoblotting analysis of cholesterol metabolism-related proteins and β-arrestin-mediated signal pathways in ARRB1/2-knockdown HepG2-Cas9 cells along with wild-type control. (a) Cells were starved and treated with TSH or AKT activator SC79 in a time course of either 1 hour or 24 hours. Protein extracts were collected and analyzed by Western blot with respective antibodies. The mature type of SREBP2 was indicated as mSREBP2. (b, c) Grayscale analysis of relative expression of mature SREBP2 and CYP7A1 was performed by ImageJ. All experiments were repeated 3 times with similar results. . Error bars are calculated as a standard error (SEM).
(a)
(b)
(c)