Figure 2: VAPA is involved in AR trafficking. (a) Representative Western blot analysis and real-time PCR showed VAPA was knocked down by shRNA transfection. , . (b) Representative Western blot analysis indicated that AR localization and trafficking in membrane and nuclear fractions were decreased in VAPA shRNA group. (c) Statistical analysis of collected data was from three repeated experiments. The left panel was for membrane fraction, and right for nucleus. The data was expressed as the fold change of AR expression level at basal condition in cells transfected with NC, and the data was showed as mean ± SEM. NC: cells transfected with the negative control vector. ; .