(a)
(b)
(c)
(d)
Figure 3: VAPA-mediated AR trafficking via vesicle transport protein OSBP. The film images showed the representative blots for AR, VAPA, and OSBP in membrane and cytoplasmic fractions, before (input, (a)) or after immunoprecipitation (co-IP, (b)) by the anti-AR antibody or anti-VAPA antibody in TM4 cells treated with 10 nM testosterone for 30 min. (c) The data analysis from three repeated experiments of panel (a). The data was expressed as the fold change of the OSBP expression in cytoplasmic fraction at basal condition, and the data was showed as mean ± SEM. (d) Collected data analysis showed the testosterone enhance AR and OSBP association, as well as the binding of VAPA and OSBP in cytoplasmic fraction, . The left panel was for the ratio of OSBP/AR (OSBP expression divided by AR expression) and the right for OSBP/VAPA (OSBP expression divided by VAPA expression). The data was expressed as the fold change of the values at basal condition, and the data was showed as mean ± SEM. ; .