Leptin Modifies the Rat Heart Performance Associated with Mitochondrial Dysfunction Independently of Its Prohypertrophic Effects

Figure 3

Determination of mitochondrial potential. (a) Representative plots of the experimental measurement of potential of control (gray) and leptin-treated (black) mitochondria during active oxidative phosphorylation. The reaction was started by the addition of glutamate/malate (G/M), and the potential was collapsed by the addition of CCCP. (b), (c), and (d) are representative plots of control and leptin-treated mitochondria after 2, 3, and 4 hours of perfusion, respectively. Panel d includes a plot of leptin-treated mitochondria treated with the mPTP inhibitor cyclosporine A (CsA). Arrows indicate the time points when the indicated additions were made. Note that the potential of leptin-treated mitochondria was neither established since the first hour of perfusion nor recovered with CsA.

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