Research Article

Metformin Regulating miR-34a Pathway to Inhibit Egr1 in Rat Mesangial Cells Cultured with High Glucose

Figure 1

The effects of high glucose on Egr1 mRNA (a) and protein (b) expression. RMCs were incubated with high glucose (30 mmol/L glucose) for 0–24 hours. mRNA expression was determined by quantitative real-time PCR, and protein expression was determined by western blotting. , versus time zero control. All results represent means  SD obtained from three independent experiments in triplicate. Egr1: early growth response factor 1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.
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