Metformin Regulating miR-34a Pathway to Inhibit Egr1 in Rat Mesangial Cells Cultured with High Glucose

Figure 2

High glucose obviously suppresses SIRT1 protein and the p-AMPKα/AMPKα ratio (by western blotting) in RMCs and increases Egr1 protein (by western blotting) in RMCs. RMCs were incubated with normal glucose (NG, 5.5 mmol/L glucose), high mannitol (HM, 5.5 mmol/L glucose + 24.5 mmol/L mannitol), or high glucose (HG, 30 mmol/L glucose). versus NG control. All results represent means ± SD obtained from three independent experiments in triplicate and normalized to GAPDH. SIRT1: sirtuin 1; Egr1: early growth response factor 1; p-AMPKα: phosphorylated adenosine monophosphate-activated protein kinase α; AMPKα: adenosine monophosphate-activated protein kinase α; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.