Investigating the Interactive Effects of Sex Steroid Hormones and Brain-Derived Neurotrophic Factor during Adolescence on Hippocampal NMDA Receptor Expression

Figure 1

Protein expression of NMDA receptors in the dorsal (DHP) and ventral (VHP) hippocampus of female sham ovariectomized + placebo (intact), ovariectomized + placebo (OVX), OVX + estradiol (E2), and OVX + progesterone- (P4-) treated mice. (a) GluN1 expression in the DHP, (b) GluN1 expression in the VHP, (c) GluN2A expression in the DHP, (d) GluN2A expression in the VHP, (e) GluN2B expression in the DHP, and (f) GluN2B expression in the VHP.  = 5–7 per treatment group. , , and . Error bars represent the standard error of the mean (SEM). Two-way ANOVA was performed. Bars with connecting horizontal lines show significant interactions (OVX × genotype), flat bars represent significant main effects of the genotype, and above the OVX bar represents post hoc significant differences between OVX and sham control, while # above hormone-treated bars represents significant differences between hormone-treated OVX groups and OVX placebo. ^#, ^##, and ^###.