Effects of Osteocalcin on Synthesis of Testosterone and INSL3 during Adult Leydig Cell Differentiation

Figure 5

3βHSD, INSL3, and GPRC6A immunoreactivity in testicular tissue samples of EDS-administered subjects (Group 2). (a) High 3βHSD expression was reported in Leydig cells of control subjects (Group 2A). (b) 3βHSD expression decreased in Leydig cells of GnRH-antagonist-administered subjects (Group 2B). (c) A decrease in 3βHSD expression was seen to continue in Leydig cells of GnRH-antagonist-and-osteocalcin-administered subjects (Group 2C). (d) Intensive 3βHSD expression was observed in Leydig cells of only-osteocalcin-administered subjects (Group 2D). (e) High INSL3 expression was detected in Leydig cells of control subjects (Group 2A). (f) INSL3 expression decreased in Leydig cells of GnRH-antagonist-administered subjects (Group 2B). (g) A decrease in INSL3 expression was reported to continue in Leydig cells of GnRH-antagonist-and-osteocalcin-administered subjects (Group 2C). (h) High INSL3 expression was observed in Leydig cells of only-osteocalcin-administered subjects (Group 2D). (i) High INSL3 expression was seen in Leydig cells of control subjects (Group 2A). (j) GPRC6A expression decreased in Leydig cells of GnRH-antagonist-administered subjects (Group 2B). (k) A decrease in GPRC6A expression was seen to continue in Leydig cells of GnRH-antagonist-and-osteocalcin-administered subjects (Group 2C). (l) High GPRC6A expression was observed in only-osteocalcin-administered subjects (Group 2D). Bar = 50 μm.