Expression of Leptin Receptor and Effects of Leptin on Papillary Thyroid Carcinoma Cells

Figure 2

Effects of leptin treatment on signaling pathways of K1 and TPC-1 cells. (a) Immunoblot analysis of β-catenin, phosphorylated AKT (p-AKT) and AKT, and phosphorylated ERK (p-ERK) and ERK in K1 and TPC-1 cells after leptin treatment. GAPDH was used as a loading control. Experiments were performed as described in Materials and Methods. (b) Densitometric analysis from a representative immunoblot of p-AKT/AKT, p-ERK/ERK, and -catenin. Values are expressed as a ratio over the loading control (arbitrarily assigned as 1).

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