miR-129-5p Inhibits Adipogenesis through Autophagy and May Be a Potential Biomarker for Obesity

Figure 3

miR-129-5p mimics inhibited browning of SVF from abdominally subcutaneous fat tissues in male mice. SVF from abdominally subcutaneous fat tissues was induced to differentiate into brown adipocytes. (a) Oil Red O staining of mature beige adipocytes. (b) Relative TG content of these cells. (c) The time course of miR-129-5p expression during normal beige adipogenic differentiation and C/EBPα, PPARγ2, and UCP1 gene expressions were quantified by RT-qPCR. (d) Marker proteins and genes of brown adipocytes were determined by western blot. (e) Densitometry quantification of western blot. Data were analyzed with Student’s t-test and is presented as mean ± SEM of a representative of more than three independent experiments (, , and ).

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