Analysis of Regulatory T Cell Subsets and Their Expression of Helios and PD-1 in Patients with Hashimoto Thyroiditis

Figure 1

Representative flow cytometric dot plots of the T cell subsets in peripheral blood of subjects. I group represent the healthy controls, II group represent HT patients, III group represent euthyroidism HT, IV group represent subclinical hypothyroidism HT, and V group represent overt hypothyroidism HT. (a) Gating Treg cells (CD25⁺Foxp3⁺/CD3⁺CD4⁺T cells), the remainder was regarded as Teffect cells. (b) Treg cells were classified into three subsets, aTreg (CD45RO⁺Foxp), sTreg (CD45RO⁺Foxp), and rTreg (CD45RO^—Foxp). (c) Teffect cells were divided into two subsets, CD45RO⁺Teffect and CD45RO^--Teffect.