The Role of Epoxyeicosatrienoic Acids in Diabetes Mellitus-Induced Impaired Vascular Relaxation of Aortic Rings in Ovariectomized Sprague-Dawley Rats

Figure 1

ACh-induced relaxation (AChIR) of isolated rat aorta rings in non-OVX-CTR and non-OVX-DM rats and OVX-CTR and OVX-DM rats. AChIR was significantly impaired in the OVX-DM group when compared to the non-OVX-DM group of rats at 10^-7-10^-5 M ACh concentration. Both non-OVX-DM and OVX-DM rats exhibited lower sensitivity to ACh compared to their corresponding controls (table within the panel). AChIR was significantly impaired in OVX-CTR compared to non-OVX-CTR (at 10^-7-10^-5 M ACh concentration) and OVX-DM compared to non-OVX-DM groups of rats (at 10^-7-10^-5 M ACh concentration). Both OVX-CTR and OVX-DM rats exhibited lower sensitivity to ACh compared to non-OVX groups, respectively (table within the panel). LogEC50 values (shown in the corresponding table) were compared by Student’s -test. : OVX-CTR vs. OVX-DM, OVX-DM vs. non-OVX-DM. : OVX-CTR vs. non-OVX-CTR. : non-OVX-CTR vs. non-OVX-DM, OVX-DM vs. non-OVX-DM. : OVX-CTR vs. OVX-DM, OVX-CTR vs. non-OVX-CTR. Ach concentration: 10^-9 to 10^-5 mol L^-1. : number of aortic rings. EC50 (mol L^-1): half maximal effective concentration presents the concentration of ACh (mol L^-1) which induces a response halfway between the baseline and maximum.