Identifying and Validating Genes with DNA Methylation Data in the Context of Biological Network for Chinese Patients with Graves’ Orbitopathy

Figure 1

Flowchart of this study. First, LMGs as DMRs with fold_ percentile and HMGs as DMRs with fold_ percentile were extracted. Second, LMG- and HMG-regulated networks were constructed based on mutual information. Third, the top 10 LMGs and HMGs were extracted as candidate genes. Fourth, for these candidate genes, the Pearson and Spearman correlations were performed to show the association between their methylation levels and clinical phenotype in the network. Finally, some candidates were selected to validate their association with GO in an enlarged population using single-cell methylation sequencing.