Immunohistochemistry and real-time PCR analysis for steroidogenic enzymes in testes of pups from female exposure to BPA at 5 μg/mL or 50 μg/mL throughout gestation. Immunohistochemical staining for StAR, CYP11A1 and 3β-HSD in control and BPA- exposed pups on PND14. (a–c) Immunolocalization of StAR in the testis. (d–f) Immunolocalization of CYP11A1 in the testis. (g–i) Immunolocalization of 3β-HSD in the testis. (j) Real-time PCR results of StAR in the testis. (k) Real-time PCR results of CYP11A1 in the testis. (l) Real-time PCR results of 3β-HSD in the testis. Data present mean ± SD. N=3 per treatment group, with each n representing a different litter from a different female. Black arrow points to Leydig cells that show positive staining. The ∗ indicates a significant difference compared to control at P < 0.05. Bar = 50 μm.