Wingless-Type MMTV Integration Site Family Member 5a Is a Key Secreted Islet Stellate Cell-Derived Product that Regulates Islet Function

Figure 3

Wnt5a protein is a key modulator of ISC-mediated regulation of islet function. (a) A significant change in the mRNA expression of Wnt5a at 72 hours was caused by transfection of ISCs with shRNAs directly targeting Wnt5a when compared to control. All data were expressed as (), and , shRNA Wnt5a-ISCs vs. ISCs transfected with control nontargeting shRNAs. (b) Islets cocultured with Wnt5a-/-db/m ISCs had significantly reduced insulin secretion compared with control. All data were expressed as (), and , shRNA Wnt5a-/--db/m ISCs vs. db/m ISCs transfected with control nontargeting shRNAs. (c) The shRNA-induced upregulation of Wnt5a expression in hyperglycaemic db/db ISCs increased the insulin secretion compared with the control. All data were expressed as (), and , shRNA Wnt5a+/+-db/db ISCs vs. db/db ISCs transfected with control nontargeting shRNAs.

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