Quantitative Analysis of the Proteome and the Succinylome in the Thyroid Tissue of High-Fat Diet-Induced Hypothyroxinemia in Rats

Figure 7

Human normal thyroid epithelial cells were isolated and cultured. The cells were treated with and without palmitic acid (PA) in the presence and absence of a desuccinylation inhibitor (NAM) for 24 hours, respectively. Mitochondrial OCRs were measured using the Seahorse XF96 analyzer (mean ± SEM, n = 7–8). OCRs related to the mitochondrial basal respiration, ATP production, maximal respiration, and spare capacity were analyzed and normalized to the corresponding total protein concentration per well, respectively, (ns , , ). Each datum was obtained from independent three days. One-way ANOVA followed by Turkey’s post hoc test was performed for multiple comparisons.

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