The effect of spexin on c-Fos like immunoreactivity in the hypothalamus of mice (a). Histogram showing the mean number of c-Fos like immunoreactivity neurons in several hypothalamic nuclei of mice after i.p. injection of NS or spexin (10 nmol/mouse). (b, c) Representative brain sections showing c-Fos expression in the AHA and SCN of mice i.p. injected with NS or spexin (10 nmol/mouse). Scale bars are equivalent to 100 μm. Data are showed as mean ± S.E.M. n = 5 per group. versus the control. 3V, third ventricle.
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