NBPF9 Gene May Be Involved in Congenital Hypopituitarism: A Whole-Genome Study of a Boy with Pituitary Stalk Interruption Syndrome and His Family

<div>Culture status of human embryonic stem cells, siRNA transfection effect, and target gene expression. (a) Human embryonic stem cells on the left showed scattered growth and human embryonic stem cells on the right gradually formed cell colonies. (b) Cells after transfection under a fluorescence microscope. (c) siRNA transfection after 24 h, with efficiency above 60%. qPCR results after silencing the expression of each gene are shown below to verify the inhibitory effect. (d) Expression of LHX3 and PITX1 by western blotting, in which LHX3 was significantly decreased after treatment with siRNA targeting <i>NBPF9</i>.</div>

International Journal of Endocrinology

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Figure 5

Figure 5: NBPF9 Gene May Be Involved in Congenital Hypopituitarism: A Whole-Genome Study of a Boy with Pituitary Stalk Interruption Syndrome and His Family 

Figure 5 | NBPF9 Gene May Be Involved in Congenital Hypopituitarism: A Whole-Genome Study of a Boy with Pituitary Stalk Interruption Syndrome and His Family 