Vitamin D Auto-/Paracrine System Is Involved in Modulation of Glucocorticoid-Induced Changes in Angiogenesis/Bone Remodeling Coupling

Figure 5

Effects of prednisolone and vitamin D₃ administration on the protein level of VEGF-A in bone tissue: (1) control; (2) prednisolone administration; (3) prednisolone and vitamin D₃ administration. Representative immunoblots (a) and quantification of VEGF-A dimer (b) and VEGF-A monomer (c) are shown. Protein levels were normalized to β-actin. Results are shown as mean ± SEM of three independent experiments done in triplicate; vs. control; vs. prednisolone administration.

(a)

(b)

(c)