Vitamins Modulate the Expression of Antioxidant Genes in Progesterone-Treated Pancreatic β Cells: Perspectives for Gestational Diabetes Management

Figure 2

DNA fragmentation in progesterone-treated cells. (a) RINm5F cells incubated with absolute ethanol (control) or 0.1, 1.0, and 100 μM progesterone by 24 h. (b) Cells were preincubated with 40 μM vitamin E or 50 μM vitamin C for 2 h and then with absolute ethanol (control) or 100 μM progesterone for 24 h. 10,000 events were analyzed, by flow cytometry, per sample. Data are presented as means ± SEM of three experiments in duplicates. indicates difference in comparison to control. $ indicates difference between preincubation with the vitamins and the treatment with only 100 μM progesterone (ρ < 0.05). PG: progesterone; Vit E: vitamin E; Vit C: vitamin C.

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