Adrenal (Pro)renin Receptor Expression and Serum Soluble (Pro)renin Receptor Concentration in Primary Aldosteronism

Figure 1

(a) Histological findings in aldosterone-producing adenoma. A and D: hematoxylin and eosin staining; B and E: immunohistochemical staining for CYP11B2; C and F: immunohistochemical staining for (P)RR; G: immunofluorescent staining for CYP11B2 (green) and DAPI (blue); H: immunofluorescent staining for (P)RR (red) and DAPI (blue); and I: immunofluorescence-merged images showing dual-labeled CYP11B2 and (P)RR (yellow) and DAPI (blue). Bar lengths: (A–C) 100 μm; (D–F) 50 μm; and (G–I) 20 μm. (P)RR: (pro)renin receptor and DAPI: 4′,6-diamidino-2-phenylindole. (b) Scatter plots showing the positive association between adrenal (P)RR and CYP11B2 expression. (c) Western blot analysis in aldosterone-producing adenoma. The intensity of bands for (P)RR and CYP11B2 is shown in each lane (depicted by a black arrow). The other bands are nonspecific bands. (d) Scatter plots showing the positive association between adrenal (P)RR and CYP11B2 protein levels measured by Western blotting results. (P)RR: (pro)renin receptor and GAPDH: glyceraldehyde 3-phosphate dehydrogenase.

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