G Protein-Coupled Estrogen Receptor 1 (GPER1) Mediates Aldosterone-Induced Endothelial Inflammation in a Mineralocorticoid Receptor-Independent Manner

Figure 3

Inflammatory response before and after treatment with different concentrations of G15: (a) level of cell culture medium IL-1β; (b) ICAM-1 mRNA expression; (c) VCAM-1 mRNA expression; (d) protein level of IL-1β from PBS to 10⁻⁹ M G15; (e) protein level of NLRP-3 from PBS to 10⁻⁹ M G15; (f) Western blot of IL-1β and NLRP-3 from PBS to 10⁻⁹ M G15. SiMR: the state after MR knockdown, aldo: aldosterone. value < 0.05 compared with PBS within the same aldosterone concentration, value < 0.01 compared with PBS within the same aldosterone concentration, value < 0.001 compared with PBS within the same aldosterone concentration. ns: nonsignificant compared with PBS within the same aldosterone concentration.

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