MEK1/2 Inhibitor (GDC0623) Promotes Osteogenic Differentiation of Primary Osteoblasts Inhibited by IL-1β through the MEK-Erk1/2 and Jak/Stat3 Pathways

Figure 3

GDC0623 treatment affected calcified nodule formation and intracellular calcium levels. (a) Mineralized nodules were stained after osteoblast induction in the osteogenic differentiation medium for 21 days (upper lane), the details of mineralized nodules (lower lane). (b) Intracellular calcium levels were assessed by the calcium assay kit after treatment with the differentiation medium for 7 days. Data are expressed as mean ± SD for n = 3.  < 0.05 versus the control group,  < 0.05 versus the IL-1β group.

(a)

(b)