CREB3 Transactivates lncRNA ZFAS1 to Promote Papillary Thyroid Carcinoma Metastasis by Modulating miR-373-3p/MMP3 Regulatory Axis

Figure 5

CREB3 transactivated ZFAS1 expression. (a) and (b) JASPAR predicted the positions of the putative CREB3 binding motif at −2,000 bp in the human ZFAS1 promoter. (c) CREB3 was found to be positively related to ZFAS1 expression in thyroid carcinoma. (d) CREB3 expression profile in primary tumor and normal tissues obtained from TCGA-THCA datasets (). (e) Ectopically expressed CREB3 induced ZFAS1 level tested by q-PCR (). (f) Luciferase reporter assay performed following cotransfection of the full-length ZFAS1 promoter or deleted ZFAS1 promoter fragments with the CREB3 expression plasmid or blank vector in TPC1 cells. (g) Luciferase activities were expressed as relative to that of the pGL3 vector (). (h) The binding between CREB3 and ZFAS1 was confirmed by q-ChIP assay; AchR was served as a negative control (). (i) Regulation model of CREB3/ZFAS1/miR-373-3p/MMP3 in EMT process.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)