Physiological Aldosterone Concentrations Are Associated with Alterations of Lipid Metabolism: Observations from the General Population

Hannich, M.; Wallaschofski, H.; Nauck, M.; Reincke, M.; Adolf, C.; Völzke, H.; Rettig, R.; Hannemann, A.

doi:https://doi.org/10.1155/2018/4128174

International Journal of Endocrinology

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Research Article | Open Access

Volume 2018 | Article ID 4128174 | https://doi.org/10.1155/2018/4128174

Physiological Aldosterone Concentrations Are Associated with Alterations of Lipid Metabolism: Observations from the General Population

M. Hannich,¹H. Wallaschofski,^1,2M. Nauck,^1,2M. Reincke,³C. Adolf,³H. Völzke,^2,4R. Rettig,⁵and A. Hannemann¹

Academic Editor: Mario Maggi

Received11 Dec 2017

Accepted15 Feb 2018

Published27 Mar 2018

Abstract

Objective. Aldosterone and high-density lipoprotein cholesterol (HDL-C) are involved in many pathophysiological processes that contribute to the development of cardiovascular diseases. Previously, associations between the concentrations of aldosterone and certain components of the lipid metabolism in the peripheral circulation were suggested, but data from the general population is sparse. We therefore aimed to assess the associations between aldosterone and HDL-C, low-density lipoprotein cholesterol (LDL-C), total cholesterol, triglycerides, or non-HDL-C in the general adult population. Methods. Data from 793 men and 938 women aged 25–85 years who participated in the first follow-up of the Study of Health in Pomerania were obtained. The associations of aldosterone with serum lipid concentrations were assessed in multivariable linear regression models adjusted for sex, age, body mass index (BMI), estimated glomerular filtration rate (eGFR), and HbA1c. Results. The linear regression models showed statistically significant positive associations of aldosterone with LDL-C (β-coefficient = 0.022, standard error = 0.010, ) and non-HDL-C (β-coefficient = 0.023, standard error = 0.009, ) as well as an inverse association of aldosterone with HDL-C (β-coefficient = −0.022, standard error = 0.011, ). Conclusions. The present data show that plasma aldosterone is positively associated with LDL-C and non-HDL-C and inversely associated with HDL-C in the general population. Our data thus suggests that aldosterone concentrations within the physiological range may be related to alterations of lipid metabolism.

1. Introduction

Cardiovascular diseases are the leading cause of death worldwide [1]. Among other factors, high low-density lipoprotein cholesterol (LDL-C) and low high-density lipoprotein cholesterol (HDL-C) concentrations are important risk factors for cardiovascular disease [2].

Aldosterone, a hormone produced in the zona glomerulosa of the adrenal glands, has a major impact on metabolic pathways and is connected to multiple cardiovascular pathologies including endothelial dysfunction, thrombosis, and myocardial as well as vascular fibrosis [3]. Several studies [4–9] suggest that there may be associations between the concentrations of aldosterone and certain components of lipid metabolism in peripheral circulation. In an experimental setup including 30 volunteers, Goodfriend et al. [6] described a negative association between aldosterone and HDL-C and a positive correlation between aldosterone and triglycerides. Several other studies [5, 7–9] described associations between aldosterone and metabolic syndrome, which comprises elevated serum HDL-C and triglyceride concentrations. Thus, in 2292 Framingham Offspring Study participants, aldosterone was positively associated with incident metabolic syndrome and inversely with changes in HDL-C [8]. Furthermore, lipid metabolism deteriorated after successful treatment of primary aldosteronism [4].

While these studies [4–9] suggest that there are associations between aldosterone and the components of lipid metabolism in certain patient groups, data from the general population is sparse. We therefore aimed to assess the associations between aldosterone and HDL-C, LDL-C, total cholesterol, triglycerides, or non-HDL-C in the general adult population.

2. Subjects and Methods

2.1. Study Population

Data were obtained from the first follow-up of the longitudinal Study of Health in Pomerania (SHIP-1). SHIP is a population-based project in the northeast of Germany. From the population registration offices, a representative sample including 7008 adults aged 20 to 79 years was selected. Baseline examinations (SHIP-0) were conducted between 1997 and 2001 including 4308 subjects. Starting in March 2002, the first five-year follow-up examination (SHIP-1) was performed. SHIP-1 was finished in July 2006 and comprised 3300 participants, who received aldosterone measurements. Details on study design and sampling methods are given elsewhere [10]. The study participants provided information on sociodemographic characteristics, lifestyle, and medical histories. They further underwent standardized physical examinations, including measurements of body height and weight and provided blood samples. Body mass index (BMI) was calculated as weight (kg)/height² (m²). Systolic and diastolic blood pressures were measured three times on the right arm of seated subjects, using a digital blood pressure monitor (HEM-705CP, Omron Corporation, Tokyo, Japan). The mean of the second and third measurements was used for statistical analyses. Hypertension was defined as systolic blood pressure ≥ 140 mmHg or diastolic blood pressure ≥ 90 mmHg or self-reported intake of antihypertensive mediation. Diabetes mellitus was defined when the participants reported a respective physician’s diagnosis and intake of antidiabetic medication and had an HbA1c ≥ 6.5% or a random glucose concentration ≥ 11.1 mmol/l. The participants’ medication was categorised according to the anatomical-therapeutic-chemical (ATC) classification code. The intake of statins was defined according to ATC code C10. The intake of antidiabetic medication was defined according to ATC code A10, oral contraceptives as ATC code G03A. The intake of medication that alters aldosterone or renin concentrations was defined as diuretics including aldosterone antagonists (ATC C03), beta blockers (ATC C07) or other antiadrenergic agents (ATC C02), calcium channel blockers (ATC C08), angiotensin converting enzyme inhibitors (ATC C09A-B), or angiotensin receptor blockers (ATC C09C-D). Of the 3300 participants, we excluded all those (overlap exists) with missing data on aldosterone or serum lipid concentrations (), missing data on BMI () or HbA1c (), subjects with renal insufficiency (defined as estimated glomerular filtration rate (eGFR) < 30 ml/min/1.73 m²), or missing information on renal function (); with aldosterone or the aldosterone-to-renin ratio outside the study-specific reference range () [11]; and with intake of statins or medication that alters aldosterone or renin concentrations (RAAS, ) and all pregnant women (). This resulted in a study population of 1731 subjects.

All participants gave written, informed consent. The study conformed to the principles of the Declaration of Helsinki and was approved by the Ethics Committee of the Board of Physicians Mecklenburg-West Pomerania at the University of Greifswald.

2.2. Laboratory Measurements

Nonfasting blood samples were taken from the cubital vein of participants in the supine position. Aldosterone and renin concentrations were measured in EDTA plasma by radioimmunometric procedures (aldosterone: Coat-A-Count Aldosterone, Siemens Healthcare Diagnostics, Eschborn, Germany; renin: Renin III generation, Cisbio Bioassay, Bagnols-sur-Cèze Cedex, France) as previously described [11]. The aldosterone-to-renin ratio was calculated by dividing aldosterone by renin concentrations. Sodium and potassium concentrations were determined in serum on the Dimension RxL (Siemens Healthcare Diagnostics, Eschborn, Germany). Serum lipid concentrations were quantified by lipoprotein electrophoresis (HDL-C and LDL-C: HELENA SAS-3 system; Helena 7 BioSciences Europe, Tyne and Wear, UK) or were measured on the Dimension RxL (Siemens Healthcare Diagnostics, Eschborn, Germany). Triglycerides were measured enzymatically and total cholesterol was determined by the CHOD-PAP method. Non-HDL-C was calculated as difference between total and HDL-C.

2.3. Statistical Analyses

General characteristics of the study population are expressed as medians with 1st–3rd quartiles (continuous data) or as proportions (categorical data) according to sex-specific quartiles of the aldosterone concentration. Group differences were tested for statistical significance with chi-squared or Kruskal-Wallis tests. The associations of aldosterone with serum lipid concentrations were assessed in multivariable linear regression models adjusted for sex, age, BMI, eGFR, and HbA1c. We further assessed the associations of the aldosterone-to-renin ratio with serum lipid concentrations to examine whether the associations are driven by aldosterone per se or by the relation of aldosterone-to-renin. For the regression analyses, we log transformed exposure and outcome variables to obtain normally distributed residuals. We report β-coefficients with standard errors and values. A value of was considered statistically significant. All statistical analyses were performed with SAS 9.4 (SAS Institute Inc., Cary, North Carolina, USA).

3. Results

The study population included 793 men and 938 women aged 25–86 years (median 47 years, 1st–3rd quartile 37–57 years). Subjects in the highest aldosterone quartile were on average four years younger than those in the lowest aldosterone quartile (42 versus 46 years, , Table 1). Further group differences in systolic or diastolic blood pressures, eGFR, HbA1c, or BMI were not statistically significant. Also, total cholesterol, LDL cholesterol, non-HDL cholesterol, and triglycerides were not significantly different in the aldosterone quartiles. In contrast, HDL-C decreased with increasing aldosterone. Thus, HDL-C was 1.22 mmol/l (1st–3rd quartile 0.95–1.55 mmol/l) in the lowest aldosterone quartile and 1.13 mmol/l (1st–3rd quartile 0.90–1.45 mmol/l) in the highest aldosterone quartile ().

The linear regression models (Figure 1) revealed positive associations of aldosterone with LDL-C (β-coefficient = 0.022, standard error = 0.010, ) and non-HDL-C (β-coefficient = 0.023, standard error = 0.009, ) as well as an inverse association of aldosterone with HDL-C (β-coefficient = −0.022, standard error = 0.011, ), while the associations with total cholesterol (β-coefficient = 0.010, standard error = 0.007, ) and triglycerides (β-coefficient = 0.029, standard error = 0.020, ) were not statistically significant. The relations of the aldosterone-to-renin ratio with the lipid concentrations pointed in the same direction but none of the examined associations was statistically significant (total cholesterol: β-coefficient = 0.009, standard error = 0.006, ; LDL-C: β-coefficient = 0.012, standard error = 0.009, ; HDL-C: β-coefficient = −0.004, standard error = 0.010, ; non-HDL-C: β-coefficient = 0.015, standard error = 0.009, ; and triglycerides: β-coefficient = 0.019, standard error = 0.018, ).

4. Discussion

The results of several cross-sectional [5, 7] and longitudinal [8, 9] studies that included patients with metabolic syndrome suggest that increased plasma aldosterone is associated with an altered lipid metabolism, including decreased HDL-C [5, 7–9] and increased triglycerides [7, 9]. Another study in 2891 Framingham Offspring Study participants [12] and without focus on the metabolic syndrome demonstrated an association between higher aldosterone and an elevated total/HDL-C ratio but not with HDL-C alone. Further lipid metabolism components were not evaluated in this study [12]. Our results confirm the previously reported inverse association of aldosterone with HDL-C [5, 7–9], while positive associations of aldosterone with triglycerides [7, 9] could not be replicated. As major new findings, our data show that plasma aldosterone is positively associated with LDL-C and non-HDL-C in the general population. As the associations of the aldosterone-to-renin ratio with lipid concentrations were not statistically significant, increasing aldosterone per se seems to control the observed associations.

Our data and the results of the abovementioned studies [5, 7–9, 12] suggest that high aldosterone may have a negative effect on circulating lipid concentrations. This in turn may contribute to the cardiovascular damage observed with inappropriately high aldosterone concentrations [13]. The detrimental effect of aldosterone is most impressively observed in patients with primary aldosteronism, a serious condition with extremely high aldosterone concentrations. Primary aldosteronism causes hypertension, and patients commonly have a high cardiovascular risk [14] and wide range of comorbidities [15]. Yet, a recent study [4] reported that successful treatment of primary aldosteronism resulted in increased serum LDL-C and triglyceride levels as well as decreased HDL-C levels. While these results seem counterintuitive at first sight, the worsening lipid profile under surgical or medical therapy might not result in a higher cardiovascular risk. Instead, the manifold therapy benefits, including a lowering of blood pressure, probably outweigh the adverse lipid changes [15]. In addition, the effects observed in primary aldosteronism may not be representative of the general population. Apart from primary aldosteronism, negative effects of increasing aldosterone have been reported in several studies, for example, in patients with chronic heart failure [16], myocardial infarction [17], or the general population [18]. In these studies [16–18], increasing aldosterone was related to an elevated cardiovascular mortality [17, 18] or incident hypertension [16]. Whether our findings of increasing LDL-C and non-HDL-C and decreasing HDL-C with increasing aldosterone contribute to incident cardiovascular events in the general population and whether a lowering of circulating aldosterone concentrations may be cardioprotective cannot be answered by our cross-sectional study. However, previous studies point into that direction [19, 20]. Thus, a combined RAAS blockade with an angiotensin-converting enzyme inhibitor and an angiotensin II AT1 receptor blocker led to increased HDL-C and decreased LDL-C levels in patients with diabetes and hypertension [20]. Moreover, treatment with mineralocorticoid receptor antagonists in addition to standard therapy improved cardiovascular morbidity and mortality in heart failure patients [19].

The mechanisms underlying the associations between circulating aldosterone and lipid concentrations are currently poorly understood, but a brought range of pathophysiological explanations has been discussed. The most evident approach relates to the link between obesity and RAAS activity. Adipose tissue, via secretion of factors such as leptin, stimulates adrenal aldosterone secretion [21]. Aldosterone, via the mineralocorticoid receptor, may in turn mediate adipocyte differentiation, thereby contributing to adipogenesis and inflammation [22–24]. Indeed, BMI and aldosterone were closely linked in our study. A linear regression model adjusted for age and sex demonstrated a positive relation between the measures (β-coefficient = 0.010, standard error = 0.004, ). Nevertheless, the associations between aldosterone and LDL-C, HDL-C, and non-HDL-C remained significant after adjustment for BMI. Another approach linking aldosterone and lipid concentrations aims at HDL-C, which may directly inhibit adrenal aldosterone secretion by modulating the sensitivity of the adrenal zona glomerulosa [6]. Other studies (reviewed by Trevisan et al. [25]) showed that high aldosterone concentrations may induce kidney injury and proteinuria. The herewith associated loss of albumin, lipoproteins, and liver proteins may affect LDL-C and HDL-C synthesis [26].

The present study stands out due to its large sample size as well as comprehensive and standardized data collection. Limitations arise from the observational study design, which implies that the results of this study cannot be interpreted in a causal way. Our cross-sectional analysis is further not suited to draw conclusions on related incident cardiovascular outcomes. Moreover, data on salt or water intake of the study participants was not collected. While we have no reason to assume that our study participants consume more than average amounts of salt or water, we also cannot exclude that single individuals may do so. Finally, insights into the pathophysiology of the topic cannot be generated by our study.

Taken together, the present data show that plasma aldosterone is positively associated with LDL-C and non-HDL-C in the general population. Furthermore, our study confirms the previously reported inverse association of plasma aldosterone with HDL-C. Our data suggest that aldosterone concentrations within the physiological range may be related to alterations of lipid metabolism.

Conflicts of Interest

The authors declare that there is no conflict of interest regarding the publication of this article.

Acknowledgments

SHIP is part of the Community Medicine Research net of the University of Greifswald, Germany, which is supported by the German Federal State of Mecklenburg-West Pomerania. INSTAND e.V. provided partial grant support for the determination of plasma samples in SHIP.

References

World Health Organization, Global Status Report on Noncummunicable Diseases 2014, WHO Press, Geneva, 2014.
S. C. Smith Jr., “Multiple risk factors for cardiovascular disease and diabetes mellitus,” The American Journal of Medicine, vol. 120, no. 3, pp. S3–S11, 2007.
View at: Publisher Site | Google Scholar
A. D. Struthers and T. M. MacDonald, “Review of aldosterone- and angiotensin II-induced target organ damage and prevention,” Cardiovascular Research, vol. 61, no. 4, pp. 663–670, 2004.
View at: Publisher Site | Google Scholar
C. Adolf, E. Asbach, A. S. Dietz et al., “Worsening of lipid metabolism after successful treatment of primary aldosteronism,” Endocrine, vol. 54, no. 1, pp. 198–205, 2016.
View at: Publisher Site | Google Scholar
M. Bochud, J. Nussberger, P. Bovet et al., “Plasma aldosterone is independently associated with the metabolic syndrome,” Hypertension, vol. 48, no. 2, pp. 239–245, 2006.
View at: Publisher Site | Google Scholar
T. L. Goodfriend, B. Egan, K. Stepniakowski, and D. L. Ball, “Relationships among plasma aldosterone, high-density lipoprotein cholesterol, and insulin in humans,” Hypertension, vol. 25, no. 1, pp. 30–36, 1995.
View at: Publisher Site | Google Scholar
A. Hannemann, C. Meisinger, M. Bidlingmaier et al., “Association of plasma aldosterone with the metabolic syndrome in two German populations,” European Journal of Endocrinology, vol. 164, no. 5, pp. 751–758, 2011.
View at: Publisher Site | Google Scholar
E. Ingelsson, M. J. Pencina, G. H. Tofler et al., “Multimarker approach to evaluate the incidence of the metabolic syndrome and longitudinal changes in metabolic risk factors: the Framingham Offspring Study,” Circulation, vol. 116, no. 9, pp. 984–992, 2007.
View at: Publisher Site | Google Scholar
S. K. Musani, R. S. Vasan, A. Bidulescu et al., “Aldosterone, C-reactive protein, and plasma B-type natriuretic peptide are associated with the development of metabolic syndrome and longitudinal changes in metabolic syndrome components: findings from the Jackson Heart Study,” Diabetes Care, vol. 36, no. 10, pp. 3084–3092, 2013.
View at: Publisher Site | Google Scholar
H. Volzke, D. Alte, C. O. Schmidt et al., “Cohort profile: the study of health in Pomerania,” International Journal of Epidemiology, vol. 40, no. 2, pp. 294–307, 2011.
View at: Publisher Site | Google Scholar
A. Hannemann, N. Friedrich, J. Lüdemann et al., “Reference intervals for aldosterone, renin, and the aldosterone-to-renin ratio in the population-based study of health in Pomerania (SHIP-1),” Hormone and Metabolic Research, vol. 42, no. 6, pp. 392–399, 2010.
View at: Publisher Site | Google Scholar
S. Kathiresan, M. G. Larson, E. J. Benjamin et al., “Clinical and genetic correlates of serum aldosterone in the community: the Framingham Heart Study,” American Journal of Hypertension, vol. 18, no. 5, pp. 657–665, 2005.
View at: Publisher Site | Google Scholar
J. W. Funder, “Aldosterone and mineralocorticoid receptors—physiology and pathophysiology,” International Journal of Molecular Sciences, vol. 18, no. 5, article 1032, 2017.
View at: Publisher Site | Google Scholar
J. W. Funder, R. M. Carey, F. Mantero et al., “The management of primary aldosteronism: case detection, diagnosis, and treatment: an endocrine society clinical practice guideline,” The Journal of Clinical Endocrinology & Metabolism, vol. 101, no. 5, pp. 1889–1916, 2016.
View at: Publisher Site | Google Scholar
M. Quinkler, E. Born-Frontsberg, and V. G. Fourkiotis, “Comorbidities in primary aldosteronism,” Hormone and Metabolic Research, vol. 42, no. 06, pp. 429–434, 2010.
View at: Publisher Site | Google Scholar
R. S. Vasan, J. C. Evans, M. G. Larson et al., “Serum aldosterone and the incidence of hypertension in nonhypertensive persons,” The New England Journal of Medicine, vol. 351, no. 1, pp. 33–41, 2004.
View at: Publisher Site | Google Scholar
G. Guder, J. Bauersachs, S. Frantz et al., “Complementary and incremental mortality risk prediction by cortisol and aldosterone in chronic heart failure,” Circulation, vol. 115, no. 13, pp. 1754–1761, 2007.
View at: Publisher Site | Google Scholar
F. Beygui, J. P. Collet, J. J. Benoliel et al., “High plasma aldosterone levels on admission are associated with death in patients presenting with acute ST-elevation myocardial infarction,” Circulation, vol. 114, no. 24, pp. 2604–2610, 2006.
View at: Publisher Site | Google Scholar
B. Pitt, F. Zannad, W. Remme et al., “The effect of spironolactone on morbidity and mortality in patients with severe heart failure,” The New England Journal of Medicine, vol. 341, no. 10, pp. 709–717, 1999.
View at: Publisher Site | Google Scholar
G. Gaudio, L. Guasti, A. Schizzarotto et al., “Changes in plasma lipids during renin-angiotensin system blockade by combination therapy (enalapril plus valsartan) in patients with diabetes and hypertension,” Journal of Cardiovascular Pharmacology, vol. 45, no. 4, pp. 362–366, 2005.
View at: Publisher Site | Google Scholar
A. Vecchiola, C. F. Lagos, C. A. Carvajal, R. Baudrand, and C. E. Fardella, “Aldosterone production and signaling dysregulation in obesity,” Current Hypertension Reports, vol. 18, no. 3, p. 20, 2016.
View at: Publisher Site | Google Scholar
A. Y. Kargi and G. Iacobellis, “Adipose tissue and adrenal glands: novel pathophysiological mechanisms and clinical applications,” International Journal of Endocrinology, vol. 2014, Article ID 614074, 8 pages, 2014.
View at: Publisher Site | Google Scholar
M. Caprio, B. Feve, A. Claes, S. Viengchareun, M. Lombes, and M. C. Zennaro, “Pivotal role of the mineralocorticoid receptor in corticosteroid-induced adipogenesis,” The FASEB Journal, vol. 21, no. 9, pp. 2185–2194, 2007.
View at: Publisher Site | Google Scholar
M. Infante, A. Armani, C. Mammi, A. Fabbri, and M. Caprio, “Impact of adrenal steroids on regulation of adipose tissue,” Comprehensive Physiology, vol. 7, no. 4, pp. 1425–1447, 2017.
View at: Publisher Site | Google Scholar
R. Trevisan, A. R. Dodesini, and G. Lepore, “Lipids and renal disease,” Journal of the American Society of Nephrology, vol. 17, Supplement 2, no. 4, pp. S145–S147, 2006.
View at: Publisher Site | Google Scholar
N. D. Vaziri, “Disorders of lipid metabolism in nephrotic syndrome: mechanisms and consequences,” Kidney International, vol. 90, no. 1, pp. 41–52, 2016.
View at: Publisher Site | Google Scholar

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Copyright © 2018 M. Hannich et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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