Hormones and Sex-Specific Transcription Factors Jointly Control Yolk Protein Synthesis in Musca domestica
Figure 3
RNA and protein expression of yp genes in and strains. (a) Anti-YP crossreactivity in hemolymph (h), fat body (fb), and ovaries (ov) of males and females of a standard strain (st.: stages of ovarian development). The doublet at 45 kD represents the products of Mdyp1 and Mdyp3, while the single band at 50 kD are products of the Mdyp2 gene. (b) Anti-YP crossreactivity in males (left panel) and females (middle panel) of the strain; and in hemolymph of males (right panel). In each h lane the hemolymph of a single adult was loaded. Animals were 4d old when the samples were taken. (c) Northern blot of standard females (left panel), standard males (right panel) and males (middle panel) probed with labeled sequences specific for Mdyp1, Mdyp2, and Mdyp3. (d) Anti-YP crossreactivity in the hemolymph of single male after injection of 20E (1 g, 10 ng: amount of 20E injected), M: size marker.
