Table of Contents
International Journal of Evolutionary Biology
Volume 2011 (2011), Article ID 921312, 11 pages
http://dx.doi.org/10.4061/2011/921312
Research Article

Sequence Analysis of SSR-Flanking Regions Identifies Genome Affinities between Pasture Grass Fungal Endophyte Taxa

1Department of Primary Industries, Biosciences Research Division, Victorian AgriBiosciences Centre, 1 Park Drive, La Trobe University Research and Development Park, Bundoora, VIC 3083, Australia
2Molecular Plant Breeding Cooperative Research Centre, La Trobe University Research and Development Park, Bundoora, VIC 3083, Australia
3Bioprotection Research Centre, P.O. Box 84, Lincoln University, Lincoln 7647, Canterbury, New Zealand
4Dairy Futures Cooperative Research Centre, La Trobe University Research and Development Park, Bundoora, VIC 3083, Australia
5La Trobe University, Bundoora, VIC 3086, Australia

Received 14 October 2010; Accepted 10 December 2010

Academic Editor: Hiromi Nishida

Copyright © 2011 Eline van Zijll de Jong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

These files provide the alignment of DNA sequences of amplicons from reference endophyte isolates obtained with primer pairs designed to EST-SSR loci NCESTA1AB04 (Appendix 1), NCESTA1FH03 (Appendix 2), NCESTA1GA07 (Appendix 3), NLESTA1GF09 (Appendix 4) and NLESTA1NF04 (Appendix 5).

Each of the haplotypes represent a consensus sequence obtained from haploid isolates by direct sequence analysis of the forward and reverse product and from heteroploid isolates by sequence analysis of multiple clones containing the product. The predicted corresponding protein sequence for the EST is shown. The SSR array is shaded in dark grey and the primer sequences are underlined. In addition, for Appendices 3 and 4, the intron present in genomic DNA sequence variants is shown below the dotted line in the EST, with the consensus splice sites shaded in light grey and the invariant 5'-GT–AG-3' motifs indicated in bold.

The isolate from which each haplotype was obtained, as well as molecular size (bp) is indicated prior to the sequence string. Isolates are as follows: 9309: N. coenophialum isolate 9309; 99201: N. coenophialum isolate 9920/1; 99202: N. coenophialum isolate 9920/2; 99203: N. coenophialum isolate 9920/3; 8907: FaTG-2 isolate 8907; 9601: N. lolii isolate 9601; Ellett: N. lolii isolate Ellett H5837; NAf6: N. lolii isolate North African 6; Vic2: N. lolii isolate Victorian 2; 9414: N. uncinatum isolate 9414; 93032: Neotyphodium isolate 9303/2; 9727: Neotyphodium isolate 9727; 9728: Neotyphodium isolate 9728; 9707: E. baconii isolate 9707; 9630: E. bromicola isolate 9630; 9401: E. clarkii isolate 9401; 9412: E. festucae isolate 9412; 9436: E. festucae isolate 9436; 9713: E. festucae isolate 9713; 9718: E. festucae isolate 9718; 9722: E. festucae isolate 9722; 9301: E. sylvatica isolate 9301; 9635: E. typhina isolate 9635.

  1. Supplementary Material