Table 5: Nucleotides at four informative sites in ITS1-ITS2 sequences of ten strains of D. lini and its sibling species (D. ohnishii and D. ogumai). Diagnostic nucleotides for DNA barcoding are indicated with an asterisk. The positions of nucleotide sites are based on the sequence of D. ogumai. Determination of each region in the sequence is based on the whole sequence of D. simulans (Z28413), ITS1: 690 bp; 5.8S: 123 bp; ITS2a: 26 bp; 2S: 30 bp; ITS2: 383 bp [26]. The partial or whole sequence of each region was obtained in D. ogumai as: ITS1: last 81 bp; 5.8S: 123 bp; ITS2a: 28 bp; 2S: 30 bp; ITS2: first 392 bp. See Table 3 for further explanations.

SpeciesNITS1 (last 81 bp)ITS2 (first 392 bp)Pylogeny

D. lini 6GTGTCAATAATAAAATNon-monophyletic
D. ohnishii 2GTGTCAATAATAAAAT/deletionNon-monophyletic
D. ogumai 2A*G*GTCAATAATAAAATT*Monophyletic